Mr. Otto used the word "building" to describe the process of creating flags.
Most of the world's micronations aren't quite as serious as Liberland appears to be, creating flags, titles, and declarations but never following through on seeking independence.
Finally, using laboratory data we created flags (1 or 0) to indicate high median laboratory values based on repeated measures for participants.
In return, schoolchildren in countries like Senegal are creating flags with their own messages, to be viewed by their counterparts in Japan.
Nail art was the fashion sensation of the 2012 Olympics, with the Minx brand creating flag designs for every nation's athletes, which were applied at nail salons in the Olympic Park.
The scFvs were subcloned into the pRS316-FLAG vectors to create pRS316-FLAG-4420, pRS316-FLAG-4420-intein, pRS316-FLAG-scFv2, pRS316-FLAG-scFv2-intein, pRS316-FLAG-GFP, pRS316-FLAG-GFP-intein, pRS316-FLAG-MR1, pRS316-FLAG-MR1-intein, and pRS316-FLAG-2224, pRS316-FLAG-2224-intein. Yeast were transformed using the LiAc/ssDNA/PEG method.
The PCR product was ligated into PstI and EcoRI sites of the mammalian expression vector pMT2 to create pMT2-FLAG-MK-STYX-FLAG.
Because the FP receptors containing two Flag epitopes or HA epitopes at the amino terminus may not localize to the cell membrane (Fujino et al., 2000), we introduced a prolactin signal peptide preceding the Flag sequence to create Pro-Flag-FP and preceding the HA sequence to create Pro-HA-altFP, according to methods previously described (Kolodziej and Young, 1991).
Six days later, Six Flags purchased SeaWorld Ohio – next to Geauga Lake – with the intent of merging the two to create Six Flags Worlds of Adventure.
