Sentence examples for create constructs from inspiring English sources
The method was successfully optimized and applied to create constructs containing promoter, RBS and/or mutant enzyme libraries.
Organ-on-a-chip engineering employs microfabrication of living tissues within microscale fluid channels to create constructs that closely mimic human organs.
This study was designed to create constructs which are cytoplasmic in the absence of ligand and nuclear in the presence of ligand, and also to regulate the amount of protein translocating to the nucleus on ligand induction.
Shawn Ashmore as Bobby Drake / Iceman A young mutant, Iceman can create constructs of ice or blasts of cold.
To create constructs I+MP, PIa, and PIb, the LI sequence was amplified by PCR and cloned into the positions indicated in Figure 1A.
We used gBlocks Gene Fragments (Integrated DNA Technologies, Leuven, Belgium) to create constructs (pGL4minP-A and pGL4minP-a) for the common and risk alleles of the rs5899787 SNP.
Synthetic biologists claimed that they could do even do more than 'just' writing; that they could create, construct, indeed, 'engineer' or 'design'artificialalifefe' (Cserer and Seiringer 2009; Hellsten and Nerlich 2011).
To determine if this system could be used to measure FRET between internal sites of RyR1, additional fusion proteins were created that contained a His10 tag at amino acid residue 1861 in divergent region 3 (DR3) [17] and GFP at either the N-terminus (to create construct GFP(1 DR3His) or at amino acid position 618 (to create construct GFP 618 DR3His (Fig. 7a)).
This cDNA was cloned behind a 35S cauliflower mosaic virus promoter in a T-DNA vector to create construct 35S::PCN-miRNAd, and transformed into Populus using an Agrobacterium-based method (Materials and Methods).
To allow subsequent 'repair' of any deletion, the LEE4 operon was amplified by long-range PCR and cloned into pIB307 to create construct pAJR162.
To create construct 35S+LI, the mature 5'UTR present in construct 35S was replaced with the 5'UTR of AtMHX that included the LI, which was amplified by PCR from the WT construct.
