Sentence examples for create construct from inspiring English sources

An animated talker with an almost incessant grin, he seems to understand how they think and wants to give them the tools to design, create, construct, and learn.

Synthetic biologists claimed that they could do even do more than 'just' writing; that they could create, construct, indeed, 'engineer' or 'design'artificialalifefe' (Cserer and Seiringer 2009; Hellsten and Nerlich 2011).

To determine if this system could be used to measure FRET between internal sites of RyR1, additional fusion proteins were created that contained a His10 tag at amino acid residue 1861 in divergent region 3 (DR3) [17] and GFP at either the N-terminus (to create construct GFP(1 DR3His) or at amino acid position 618 (to create construct GFP 618 DR3His (Fig. 7a)).

This cDNA was cloned behind a 35S cauliflower mosaic virus promoter in a T-DNA vector to create construct 35S::PCN-miRNAd, and transformed into Populus using an Agrobacterium-based method (Materials and Methods).

To down-regulate PCN transcripts levels, an artificial miRNA [37], [38] was designed to target both PCN and its paralog, Pt-ATHB.11, and cloned behind the 35S promoter in a T-DNA vector to create construct 35S::miRNA-PCN, which was then transformed into Populus (Materials and Methods).

To allow subsequent 'repair' of any deletion, the LEE4 operon was amplified by long-range PCR and cloned into pIB307 to create construct pAJR162.

The PCR fragment was sub-cloned into pGEMTeasy vector (Promega), and ligated to pGL3-basic (SmaI site), creating construct 375a, or to TK-luc.

Grafting with allograft is well established, but there are problems with availability, cost, infection, antigenicity, reproducibility, and stability of the created construct.

So many of the things that make Washington one of the hottest cities to live in — our grand monuments, the elegant housing stock, the culture — were created, constructed and nurtured by its African American residents.

In creating constructed novel ecosystems within the OCBIL landscapes of Western Australia, the techniques within a baroque toolbox succour the local by embracing and enhancing intrinsic ephemera and site specificities.

Authentic learning at its core is about doing, creating, constructing.