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To achieve this, we create and assay replicated mitonuclear introgression lines.
The resultant PCR product was purified and cloned into a protein expression vector The approach used to create and assay protein production from pGEX3X- pvLDH 35-305aa was very similar to that used to create from pRSETA- pvLDH 1-43aa.
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To test this possibility, a point mutant D57I was created and assayed for its ability to hydrolyse GTP.
To test this hypothesis, we created and assayed a series of N- and C-terminal HCF-1 Basic region deletions of increasing size as shown in Figure 2.
CY created and assayed the expression of AtCNGC10-GFP fusions in protoplasts.
Knowledge of the requisite steps for effective development and optimization of PCR is needed to overcome multiplexing challenges and to successfully create and troubleshoot multiplex assays.
Qiagen creates sample and assay technologies for use in research, applied testing, and molecular diagnostics and has about 2,600 employees worldwide.
As these linkers may function as potential "output" regions for NTD-mediated allostery, we created linker mutations and assayed their effect on AMPAR function.
To date, biological components have been incorporated into MEMS devices to create cell-based sensors and assays, motors and actuators, and pumps.
Deletion strains were assigned a number (DS1-30) to create a "blind" assay for the measurement of PCA signal.
Each deletion strain was assigned a number to create a "blind" assay in which all steps of the screen were performed without prior knowledge of the gene name or function.
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