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The other missense mutants remove or create additional charged residues within the hydrophobic ligand-binding cavity.
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Here, we present a proof of concept study in which we tune these interactions in the device by deliberately doping our devices with fullerene, creating additional charge transfer states (CTS).
To investigate the I524-G202 interaction in greater detail, we created several additional charged mutants of I524 (I524R, I524N, I524N) as well as G202 (G202R, G202N, G202E, G202E) and evaluated the functional interaction of the different combinations (Table 3).
Another big selling point for Assistly was its pricing model, which included a "Flex" pricing model, which allowed users to buy usage time for users who are not full-time agents (like execs, developers, marketing, etc)., with the ability to create additional Flex seats at no charge per seat.
Broadcasters can instead choose to offer on a free and ad-supported model, with the option to create additional revenue through a one-off charge for a dedicated iPhone app, for example.
"It will not create additional business.
Congress should create additional safeguards.
This could create additional revenue for states.
Among Pulskamp's ideas for creating additional revenue were charging parking fees, implementing franchise fees on private-sector waste haulers and increasing hotel taxes, an idea that fell flat with Councilman David Gordon.
A possible explanation for this effect is that the increased net negative charge disorders the HDL particle surface, thereby creating additional free space into which apoA-I molecules can adsorb.
Several players created additional tributes.
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