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If oligo directed concatemerization resulted from homologous recombination, then repair of distinct AAV genomes would create a junction corresponding to the sequence of the oligo.
The minimum duplication length to create a junction contig rather that a full-length contig, as well as the length of the junction contigs produced, will depend on both the read and fragment lengths sequenced, and the assembly algorithm and parameters used.
The combination of zero repeats paired with 17 repeats creates a perfectly paired three-way junction; one repeat across from 17 repeats leaves one unpaired nucleotide at the junction, and two repeats across from 17 repeats can create a junction with zero to two unpaired nucleotides.
Create a junction between overlapping wires by clicking on the intersection.
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Baldessari's project not only enlisted the spectator in making meaning; it created a junction between the living and the dead.
Applying opposite charges to the tubes and the electrode causes the tubes to bend down, creating a junction that represents a 1. Applying like charges forces them apart into the 0 state.
Albany is literally at the crossroad of I-87 and I-90, creating a junction between Buffalo and Boston, and Montreal and New York.
Two DNA ends can be designed to be flanked by a type IIs restriction site such that digestion of the fragments removes the enzyme recognition sites and generates ends with complementary 4 nt overhangs; such ends can be ligated seamlessly, creating a junction that lacks the original site (Fig. 1A).
This deletion removes 267 amino acids from the extracellular domain, creating a junction site between exons 1 and 8 and a new glycine residue.
By contrast, the sub-full-length CAD transcripts found in poorly differentiated HCC cells, Mahlavu and SK-Hep1, corresponded to the absence of exons 3 to 6 or 5 and created a junction between exon 2 to exon 7 or 6, respectively.
Novel SDDs with continuous junctions on both sides are fabricated using pure boron (PureB) depositions to create a shallow junction in the entrance window side and a continuous rectifying junction with different potentials as function of the drift coordinate in the device side.
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