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Screening isolates for CRE: 1. Disk diffusion: this was detected by using imipenem, meropenem disk diffusion method.
Grizzly bear mortalities were concentrated in three main regions of the CRE: (1) Lake Louise; (2) Banff town site; and (3) Alberta Provincial lands near the Red Deer River.
This results in a cell clone called DT40 Cre 1.
The MerCreMer mRNA is constitutively expressed in DT40 Cre 1, but the protein is inactive in the absence of tamoxifen due to the fused HBD.
Neurons were infected with adenoviruses encoding GFP or Cre 1 h prior to incubating the cells with recombinant shRNA lentivirus targeting the murine bad or bim genes.
Both alleles of activation induced cytidine deaminase (AID [ 9, 10]) gene were disrupted in DT40 Cre 1 by knockout constructs which are derived from pLoxPuro and pLoxBsr.
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ACTA1-Cre::LSL- Ctnnb1, ACTA1-Cre::LSL- Ctnnb1TV-AA, and ACTA1-Cre::LSL- Ctnnb1∆TAD mice were viable and fertile and exhibited normal NMJ morphology, compared to control mice (ACTA1-Cre or Ctnnb1fl/fl) (data not shown).
Primary antibodies for CC-1 (1∶10, Abcam, MA), GFAP (1∶500, chemicon), Ng2 (1∶250, Millipore), NeuN (1∶100, Chemicon), and Cre (1∶100, Novagen) were diluted in TBLS and incubation was for 1 hour at RT and overnight at 4°C, respectively.
Cre(R292A) was virtually nonreactive towards the P-half-site.
Both Opn4 Cre and Opn4 Cre ;R26 iDTR were described in Hatori et al. (2008).
HC, His6C, HNC, and HT7C were similarly constructed by PCR amplification, using primers Cre #2, Cre #3, Cre #4 and Cre #5, respectively, together with Cre-stop-HindIII primer.
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