Sentence examples for cracking buffer from inspiring English sources

In both the cases, agarose was centrifuged at 5000 g for 5 min at 4°C, washed thrice with the assay buffer, boiled with cracking buffer and resolved on 12.5% SDS-PAGE.

Cells were harvested and were lysed in cracking buffer (0.01 M phosphate [pH 7.4]. 8 M Urea, 1% SDS, and 1% β-mercaptoethanol).

Then, the cells were resuspended in cracking buffer (Tris-HCl pH 7.5, 25.0 mM and Dithiothreitol (DTT) 1.0 mM) and disrupted by 7 minutes of intermittent sonication carried out in ice bath.

Briefly, PPD-B preparation was resuspended in cracking buffer (SDS 2%, Tris HCl 0.125 M pH 6.8, 2-mercaptoethanol 1%, bromophenol blue 0.02%, and glycerol 10%), heated for 5 min in boiling water, and resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis 12% (SDS-PAGE).

Cells were harvested by centrifugation (2000× g, 5 min) and resuspended in 100 μl yeast cracking buffer (40 mM Tris HCl [pH 6.8], 8 M Urea, 5% [wt/vol] SDS, 0.1 mM Na2-EDTA, 0.4 mg/ml bromophenol blue, 0.1% [vol/vol] β-mercaptoethanol, 7% [vol/vol] benzamidine, and 5% [vol/vol] PMSF) per 7.5 OD units (e.g., 37.5 OD-units/7.5 = 500 μl YCB).

The government produced failure and insecurity, and crack buffered the results (and proved a boon to a burgeoning prison-industrial complex).

After incubation, agarose beads were washed in lysing buffer and cracked in loading buffer.

In addition to its rheological properties, the enamel of unerupted crowns experiences relatively limited cracking and flaking because the structure is buffered against the direct effects of heat by the surrounding bone of the jaw [39] [41].

Leaving a small barrier around the side of the room will give the flooring enough of a buffer to do its thing without cracking.[1].

As a result, AISI 316L stainless steel clad with a KMS 309L buffer layer showed a significant reduction in susceptibility to hot cracking.

Delamination and/or cracks were created at the interface between the bond and top coats or above the interface in the single-layer TBCs, but the TBCs with the buffer layer were delaminated and/or cracked at the interface between the buffer layer and the top coat, independent of buffer layer species.