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Accordingly, the cDNAs of EBV Rta M-ABAA strain) and KSHV RTA (Genebank: U71367.1) were PCR-amplified with CPO I sites flanking at both ends, and subcloned into pLenti4-Flag-CPO.
In brief, the original attR1 site to V5 epitope region (nt 2405 4203) in pLenti4/TO/V5-DEST was replaced with an in-frame DNA fragment encoding Kozak sequence, ATG, FLAG tag and a rare cutter CPO I site (5'CGGTCCG).
A Cpo I and a Not I restriction sites were introduced to the P1 and P4 respectively for cloning purpose.
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Enzymatic oxidative decolorization of soluble aniline blue was employed to evaluate the catalytic performance of immobilized CPO (I-CPO).
One CPO wrote: 'I have been assisting at the medical outpatient department to care for accident cases and other emergencies'.
The decolorization efficiency of dye by I-CPO was higher than 90% within 10 min.
The relative activities of I-CPO in the 20 times use maintained 62% of that in the first run.
Moreover, I-CPO displayed improved thermostability and kept higher activity in wider pH range and in the presence of high concentration of oxidant (H2O2) compared to that of free enzyme.
In this work, we employ this technology to arrange four achiral nanoparticles with different sizes in a concentric circle, and in this case a kind of new CPS, i.e., CPO, can be formed on substrate, as shown in Fig. 1a, b.
Cpd I did not accumulate sufficiently to be observed by EPR during decay of the β-chain hydroperoxo ferric intermediate at lower pH, and under no conditions was Cpd I observed for HRP, CPO, α-chains, or Mb, even though Cpd I of HRP and CPO are relatively stable at 200 K, the temperature at which decay of the hydroperoxy intermediates is complete.
Definition 3 A game Γ has extended weak general complementarities on CPOs (hence, is a GEWGC) if (i) each strategy set X i (a subset of a complete lattice Y) is a CPO; (ii) ∀ x ∈ X : the joint best reply correspondence F is nonempty and has a least element; and (iii) F is lower increasing.
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