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Two state-of-the-art methods, BBN [11] and CPL [12], are implemented for comparison.
For fixed-radius plots the optimal strategy of 6 m radius was very clear (CPL 20.72).
Former England batsman Kevin Pietersen has entered the draft for the annual Caribbean Premier League (CPL) Twenty20 tournament.
Photomicrograph detail (2 × 2 mm) of CPL 4, 1941.
Know if the case is a Misdemeanor, Offense, or Felony and whether the felony is pre or post indicted to know whether you are filing a CPL 240.20 or a CPL 190.50 demand.
Figure 1 Non-reducing SDS-PAGE of Cpl-1 C45S;D194C PEGylated with PEG 40K.
The production and purification of all proteins followed a protocol that has already been described for Cpl-1 (Loeffler and Fischetti, 2003) and Cpl-1 mutants (Resch et al., 2011).
The nucleotide sequence of Cpl-1 can be access from the Genbank database with accession number NC_001825.
In the present study, seven mutants previously described elsewhere as showing comparable antimicrobial activity to parent Cpl-1 were included (Resch et al., 2011).
While introducing cysteines at several sites on Cpl-1 did not alter its bactericidal activity, PEGylation on these residues totally abrogated it.
Plasmids carrying the genes encoding for the Cpl-1 mutants included in this study were constructed as described elsewhere (Resch et al., 2011).
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