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The crystal structures of wild-type CphA and its N220G mutant show the structural features of the active site of this enzyme, which is modeled specifically for carbapenem hydrolysis.
Most genera of cyanobacteria (Simon 1987; Allen 1988; Mackerras et al. 1990; Liotenberg et al. 1996; Wingard et al. 2002) and also some heterotrophic bacteria (Krehenbrink et al. 2002; Ziegler et al. 2002; Füser and Steinbüchel 2007) harbor a cyanophycin synthetase gene (cphA) and are able to synthesize CGP.
However, the closed SPM-1 structure differs from the CphA, and other B2 structures, in that α3 is extended by 7 residues and by the presence of the α4 helix.
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Beside those chromosomally located in environmental bacteria (Bacillus cereus-BCI, BCII, Aeromonas spp.-CphA, and S. maltophilia-L1), acquired MBL encoding genes are often located in gene cassettes within integron, being part of a plasmid or chromosome.
Furthermore, there are occasional workflow issues in the clinic that have hindered completion of cPHAs and some patients' health prevents them from complying.
Unusually, SPM-1 has high sequence similarity with MBLs from both B1 (35% identity with IMP-1 and 30% with BcII) and B2 (32% with ImiS and CphA) MBL subclasses.
Three β-lactamase genes, ampC (ASA_1191), ampS (ASA_4346) and cphA (ASA_3612), previously described in A. sobria (as cepS, ampS and imiS, respectively) [ 57] are carried on the A449 chromosome (Table 3).
Excluding the patients with the extreme values of 0 and 10 represented only approximately 3%% patients with both iPHA and cPHA surveys in their clinical record.
Those patients with both iPHA and cPHA during this period had completed approximately 31,600 cPHAs over varying numbers of subsequent visits.
Therefore, within-patient pain outcomes were examined in the patient population that had both iPHA and cPHA responses on file from the April 2010 to September 2014 time period.
This was due to relatively weak loading values (between 0.4 and 0.5) in each of these two cases, in both the iPHA and cPHA analyses.
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