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HCT-8 cultures were switched to Cp media approximately 18 hours prior to infection.
Following a 2 h excystation period at 37°C, cells were washed repeatedly with warm HBSS and incubated at 37°C in fresh Cp media until harvested.
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Western blot analysis of CP vs. NP media confirmed overexpression of AGR2 protein in cancer.
Anaerobic sludge was supplemented with 2-CP and nutrient media in a batch reactor to obtain a 2-CP acclimated culture.
Evaporation and cellular uptake of CPs from culture media are concurrent events.
Losses of CPs from culture media without cells were determined over the intended incubation duration of 180 min.
Based upon voltammetric experiments in anhydrous, acid, basic and neutral, partially aqueous media, on cp.
Carotid ultrasound (MicroMaxx, SonoSite with the linear transducer HFL38/13-6 measuredsured the presence of carotid plaques (CP) and carotid intima-media thickness (IMT; semi-automated, FDA-approved software, Sono Calc IMT®), as previously described [ 28].
According to the surface chemical analyses, the equivalent circuits simulating the electrochemical behavior of strained CP titanium in slightly acidic media with different NaF concentrations were proposed.
Reasons why adolescents with CP communicate less by virtual media could be that they are more often supervised by adults or that they have poorer motor skills.
This decrease in performance can be partly explained by a lower benefit of increased media consumption in CPs compared to controls.
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