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The species at 1530.7701 covering the sequence 186 198 of P0 is not an artifact and might have a functional role in the myelin architecture.
Where the software repeatedly misassembled joints even after the addition of PCR patches due to ubiquitous repeat sequences, longer artificial reads covering the sequence flanking the repeat were used to guide the assembly.
No other peptides covering the sequence of KDM1A displayed changes in deuterium uptake upon peptide addition (an example is shown in Figure 7A,C).
The obtained peptide coverages of the fragments (total 55 peptides covering amino acid 254 1331 for the upper fragment and 38 peptides covering the sequence from amino acid 476 1331 for the lower fragment) suggest that both fragments have similar C-terminal end but different N-terminus.
The FISH assays were developed based on bacterial artificial chromosome (BAC) DNA fluorescent probes covering the sequence of the RRM1 gene at 11p15.5 (BAC clones RP11-23F23 and RP11-982G19) and the RRM2B gene at 8q22.3 (BAC clone RP11-318G5), respectively.
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Twenty-mer peptides (with 11 amino acid overlaps) covering the sequences of H and F were synthesized using solid-phase peptide chemistry by the JHU Synthesis and Sequencing Facility and peptides for each protein were pooled.
Within the 3.986 kb of SRF1 genomic sequence covering the coding sequence we observed a total of 78 L er/Col polymorphisms or about 20 polymorphisms per 1 kb.
RepA of pPDL2 has more than 50% identity covering the complete sequence of RepA sequences of the IncP group of plasmids, pADP-1, pEST4011, and pUB1 (Suzuki et al. 2010).
The sequences for the PCR primers covering the coding sequence for six exons were provided by Dr Carpten (the National Human Genome Research Institute, National Institutes of Health).
If this resulted in a perfect hit to the sequence of the originally predicted gene in strain M1 covering the entire sequence in the nr database without any in-frame stop codons, the strain M1 annotations were updated.
The sequence is divided into equal-sized non-overlapping windows covering the whole sequence (Fig. 2).
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