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Silva, J.M. et al. Second-generation shRNA libraries covering the mouse and human genomes.
A total of ∼ 40 contiguous 1.5-mm-thick transversal slices covering the mouse body were recorded.
Extracted DNA was then analysed by qPCR with primers covering the mouse Cdx2 proximal promoter (table 1) and the values normalised against the IgG negative control.
4C-seq patterns were corrected vs previously generated random 4C seq libraries (Noordermeer et al., 2011), consisting of BACs covering the mouse Hox clusters (HoxD: RP23-331E7; HoxC: RP23-430C12; HoxB: RP23-381I12 and RP23-196F5; HoxA: RP24-298M24).
These ions are accelerated to energies of 500 eV, formed into a beam, focused to 1 mm diameter onto the tissue sections, and electrically rastered over an area of 400 mm (20 × 20), thus covering the mouse brain coronal tissue surface.
Check to make sure no glue is covering the mouse's nose.
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First, the use of PB transposon mutagens allowed us to quickly generate a large collection of iPSCs with different insertions covering the entire mouse genome.
Recently, we have systematically screened a large set of overlapping synthetic peptides covering the whole mouse laminin-1 for their cell adhesive activities.
To verify the expression of genes identified on our cDNA array, and to sample a more complete set of genes covering the whole mouse genome, we purchased additional microarrays from Agilent technologies.
The International Mouse Phenotyping Consortium is coordinating efforts to phenotype single gene knockouts covering the entire mouse genome, including characterising developmental defects for those knockout lines that prove to be embryonic lethal.
The potential of EP lethal lines for advancing our understanding of embryo development and the genetics of developmental disease is easy to grasp; but the sheer scale of the opportunity afforded by a knockout screen covering the entire mouse genome is potentially daunting.
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CEO of Professional Science Editing for Scientists @ prosciediting.com