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Consistent with our previous work in mammals (Ingolia et al., 2011) and yeast (Ingolia et al., 2009; Brar et al., 2012), many 5′ UTRs in Drosophila contain substantial footprint density covering sequences that appear to be upstream open reading frames (uORFs; example in Figure 2C).
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High quality sequence data covering the target SFP probe regions were obtained for 189 of these, while partial sequences that did not extend into the target regions were obtained for an additional 21 loci.
The first ten days of filming took place on the Serchio River, covering the battle sequence that opens the film.
As of November 2015, PubChem contains more than 150 million depositor-provided substance descriptions, 60 million unique chemical structures, and 225 million biological activity test results (from over 1 million assay experiments performed on more than 2 million small-molecules covering almost 10,000 unique protein target sequences that correspond to more than 5000 genes).
Since we have chosen the reference sequences randomly in our analysis, we can safely assume that the majority of the sequences that we have studied fold into a common structure, and that therefore the shape-covering theorem applies.
Then, he sequences that.
Regardless of the simulated average coverage, they were the outcome of random errors in sequencing that could not be corrected by additional reads covering the same position.
Clones covering defined Idd candidate regions were selected from either the DIL library or the CHORI-29 library using the BAC end-sequences that had been aligned to the Mouse Genome Sequencing Consortium v3 (mouse3) mouse reference genome in Ensembl (15).
From the 172 SNP loci we selected 22 loci with high coverage [all five strains had at least 100 sequencing reads covering that base position (Table S6)].
The sequencer generates n reads covering that locus.
We then computed, for each position along the sequence of interest, the number of inserts covering that position.
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CEO of Professional Science Editing for Scientists @ prosciediting.com