Exact(4)
Subsequently, the microplate was covered with sealing film and incubated at 4 °C for 24 h.
The plates were covered with sealing tape and briefly vortexed to break up the worm pellet.
Aliquots of 100 μl of diluted protein samples were added into wells of the microwell strips (mounted in a 96 well microplate frame), which were subsequently covered with sealing tape and allowed to incubate for 2 h at 37 °C.
The plate was covered with sealing film and heated to either 55°C or 70°C for 240 min and to 94°C for 10 min in a thermocycler.
Similar(56)
After the print run was completed, oligo plates were covered with seals and deep frozen at -20°C in a protected environment.
A drop of mineral oil was added on each well to prevent the mixture from evaporating and the plate was covered with PCR sealing film.
The plate was then covered with the sealing film, and was incubated at 17°C for 18 hr without shaking.
The aggregate was covered with a sealing layer of Matrigel mix and incubated at 37°C for polymerization after which DMEM with 10% FCS and pen/strep supplements was added.
The plate was covered with aluminum sealing tape (Corning) and the cells were grown at 25°C for 5 days to allow for protein depletion.
qPCR was performed using an Applied Biosystems 7300 Real Time PCR System in an optical 96-well plate covered with optical sealing tape (Applied Biosystems, Foster City, California, USA).
Immediately following the 2-hour incubation period, an additional 100 μl of dilution media was added, and plates were covered with adhesive sealing film (Excel Scientific, Victorville, CA) and returned to 37°C under 5% CO2.
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