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Many EEM parameter sets have been published in literature, and are available in ACC as built-in sets [28, 34, 67 70] with full information regarding the parameter development procedure (atom types covered, target molecules, QM reference data, literature reference).
While captured fragments extending the tiled regions increase the fraction of sequence covered target parts, it is decreased by ineffective probes.
Bisulfite sequencing primers were designed with Methyl Primer Express® v.1.0 (Applied Biosystems) so that the amplicons covered target CpG sites.
The survey covered target and control groups for UNICEF's DFID-funded Sanitation and Hygiene Education and Water Supply in Bangladesh (SHEWA-B) programme, including hard-core poor, those from the poorest families.
Therefore, a panel was chosen that covered target genes of RAS/RAF/MEK/ERK signaling (FOS, DUSP5), stimulators (cyclin D1/ CCND1), and inhibitors (CDKN1A, TP53) of cell cycle progression as well as proapoptotic (BAX, FASLG) and antiapoptotic (BCL2) effectors.
They also propose the MKDSC (maximum K directional sensor cover) algorithm selecting and assigning directions for a subset of K sensors such that the number of covered target points is maximized.
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For each aggregate data set, 20 22 Gb high quality reads covered targeted WES regions; each targeted base had an average of 179 - 208x coverage.
Roethlisberger threw a pair of interceptions deep in Pittsburgh territory – both poorly thrown balls to heavily covered targets – that helped the Ravens take control.
"The superior precision is also very useful against small or partially covered targets at shorter range," Kel-Tec said on its Web site.
This methodology takes into account the association of the sensors with the poorly covered targets that set an upper bound on the overall computed lifetime.
The player can take cover, target a specific person or animal, blindfire, and free aim.
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