15 µl cells in TAP media were applied to cover glass and inverted onto slides.
Cover glass dish with sauce.
Following washing, the sections were covered with cover glasses using aqueous mounting medium (Immuotech, Marseilles, France).
All samples were mounted on glass slides and covered with glass cover slides for observation.
Microscope cover glasses were then mounted on microscope slides.
Cells (10.000 cells/cover glass) were seeded on sterile cover glasses (12 mm in diameter, NeoLab, Heidelberg, Germany).
Sections were mounted on slides, stained with hematoxylin-eosine and covered with cover glasses.
HepG2 cells on cover glasses were incubated and then fixed with 10% formalin.
Silanization of cover glasses and preparation of flow-cells was previously described [11].
Transfected cells were plated on cover glasses pretreated with rat tail collagen (Roche Applied Sciences).
Cells transfected with various GFP fusion proteins were grown on 11 mm diameter cover glasses.
