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Table 2 shows the total sequence length (with a minimum of 6X coverage) used for SNP mining in each pool, ranging from 4.4 Mb (pool 6, group melo) to 15.7 Mb (pool 4, group momordica).
> Because the coverage used for this analysis was relatively low, it was necessary to establish the extent of variation in the number of gene-RT MPR (i.e., the extent of proximity of LTR-RTs to genes) determined by the stochasticity in read packages used for mapping.
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Only genes with ≥ 70% coverage were used for gene-related calculation, and only CpG positions with ≥ 5X coverage were used for gene body methylation level estimation.
Various coverage metrics used for measuring the explored extent of verification also are provided.
In particular, the same amount of smoothing and damping and the same path coverage are used for a given period.
P. reichenowi contig sequences contained some regions of single-read coverage, so nucleotide mismatches in regions of single-read coverage were edited to match the P. falciparum sequence, and only well supported nucleotide mismatches in regions of multiple-read coverage were used for analysis.
For the novel viral case, approximately 1× coverage was used for each viral genome.
Only regions with above 80% sequencing coverage were used for further analysis.
Only non-TE gene models at ≥50% coverage were used for further analysis.
This resulted in the creation of five independent contig coverage matrix, used for the clustering process.
Only regions of the genome with at least a 10x threshold of genome coverage were used for SNP search.
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