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The results of performance evaluations, carried out in ns-3 [20], show that our proposed TCDC system achieves better performances compared to state-of-the-art protocols in terms of the number of active sensing nodes, sensing coverage, standard deviation of residual energy, and network lifetime.
We find that even with as little as 2x coverage (standard deviation 0.5), and 1,000 selected SNPs (from 189k SNPs) PoolHap is able to estimate the haplotype frequencies to within 2% of their true value (Figure 1(b)).
Results are only slightly better when coverage standard deviation is reduced to zero (Figure 1(a)), indicating that PoolHap algorithm is free of stochastic coverage which frequently occur with NGS.
The output format for counts and coverage information across reference sequences is a simple tab separated file with fields for the reference sequence, annotation start/stop coordinates, counts, median coverage, mean coverage, and coverage standard deviation (see Additional file 1 for a detailed example).
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For the BM panel, with a 5-fold coverage the standard deviation of coverage values across unamplified BL and SCL samples was σ = 0.013 and σ = 0.036, respectively, suggesting little effect of input quantity.
The average coverage by enzyme combination and standard deviation (Coverage ± SD) are indicated, as are the minimum and maximum cDNA pool coverages for individual enzyme combinations (Min-Max Coverage).
On average each of the 64 amplicons (grouping forward and reverse reads) had 85× coverage with a standard deviation of 32 (Table 2).
We set a threshold for mean deletion coverage plus one standard deviation (2.1×) to bring any individual to further analysis.
In order to assess the effect of different starting DNA quantities on target coverage, we calculated the standard deviation between samples at differing coverage levels.
To quantify the volatility of the relative reads coverage, we used the standard deviation of the difference between the observed and fitted relative reads coverage.
In the first round, all mapped RAD loci with coverage higher than one and lower than the individual cutoff (i.e., mean coverage plus two times standard deviation) were used, and the second round excluded RAD loci with either more than 5%% variable sites, or more than 4 variable sites segregated in 10bp fragment, or more than 20%% sequence divergence from the reference genome.
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