Your English writing platform
Free sign upSuggestions(1)
Exact(4)
As we wrote in our initial coverage of the position, it's not surprising that Twitter would want a presence in the Beltway.
This limitation might be overcome by deeper sequencing (higher coverage of the position in question).
The number of reads mapped to a specific position (nucleotide) in the reference genome is denoted as "coverage" of the position in this paper.
Our criteria for identifying sites of potential heteroplasmy in the mitochondrial genome included >200× coverage of the position with high-quality bases, and the observation of more than one allele at >5% frequency (that is, at least ten high-quality observations of the alternative allele).
Similar(56)
PCR artifacts were ruled out by repeated experiments to increase the coverage of the positions.
The SAM file produced by Bowtie was processed using SAM tools (Li et al. 2009) to obtain the mean coverage of the positions covered by reads.
Our sequencing achieved 23 × coverage of this position of the genome as well as an average base quality of 35.22±2.04.
For each SNP found in the Sanger reads, we then asked if there was coverage of this position in the RNA-Seq data, and if there was coverage whether we had detected the SNP.
Note that the coverage depth of the position (number of reads) does not appear in the equation, but it does to calculate the maximum number of false methylcytosines.
A comparison of the coverage of the bait/oligonucleotide positions of the available CCDS-based exome sets and the GENCODE exome with the set of GENCODE design targets (Table 1) illustrates the increased coverage of our extended target set.
Absence of sequence coverage at the position of an SNP in one or two of the sequences is denoted in Table S3.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.
Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com