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The sequencing depth of indigenous populations (estimated based on coverage of reference genomes) varied significantly across the five geothermal sites, tracking inversely with microbial diversity.
The qualified reads was then used to calculate the overall coverage of reference barcodes using SOAP coverage [ 61].
Further, it allows SNP and DIP detection, RNA secondary structure prediction and analyzing the coverage of reference features and the whole reference sequence.
A total number of 18.3 million of 100-bp paired-end reads were generated, which represented more than 150-fold coverage of reference genome of the laboratory strain S288c (table 1).
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While the database contains the complete contents of all the major journals and many minor ones as well, its coverage of references and citations is much less complete.
This could be caused by low sequence coverage of the reference genome, reference errors, sequencing errors and defined mapping criterions.
Proportion of mean coverage is a measure relative to the depth of coverage of the reference reads mapped backed to the reference assembly.
We set the parameters of ART to generate target DNA fragments by randomly sampling the reference sequences until a 10-fold coverage of the reference dataset was reached.
Mapping the non-human reads from each isolate against the reference isolate CWL029 resulted in 93.7%-99.9% coverage of the reference genome (Table 2).
Two sequencing libraries on SOLiD platforms yielded over 865 million reads, and combined alignment to the domestic sheep reference resulted in a nearly complete sequence (95% coverage of the reference) at an average of 12x read depth (104 SD).
Approaches for increasing the number of loci with SNPs could involve mapping more reads to our reference transcriptome (n = 25,002 isogroups), increasing the coverage of our reference transcriptome (yielding perhaps 30,000 to 40,000 loci), and relying on genomic sequencing to develop additional markers in non-transcribed regions.
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