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In addition, we applied a coverage filter at the genotype level to exclude calls with coverage below 2 or above 250.
Program parameters were varied in repeated runs: CYNTENATOR gap and mismatch penalty parameters were set to 0.1, 0.5, 1, 2, 3, 5, 20, the alignment score threshold was set to 1, the minimal alignment length to 2, the maximal number of retained alignments to 1,000 and the gene coverage filter to 4. MCMuSeC was run to report gene clusters, which are shared by at least two genomes.
All transcripts passed coverage filter were included in this network.
The frequency of such assignments is reduced by application of the length coverage filter.
Moreover, the number of SNPs decreases as the depth of coverage filter is increased.
An 8 fold coverage filter on contigs was used in Eucalyptus, and a 2× minimum coverage in S. alba.
Similar(43)
To minimize sampling biases, the combined dataset was subjected to coverage filters for missing data.
As a consequence, although filtering will be beneficial at high coverage, at low coverage filtering will be detrimental.
For 1 N relations, the coverage filters are a bit more stringent that above: 0.8 <=sf_length/cdd_length < = 1.25 sf_coverage > = 0.75 cdd_coverage > = 0.75 1957 1 N relations were filtered out.
This step also captures partial sequences (entries labeled as fragments in UniProt) which were approved by the coverage filtering applied (see Methods for details).
We chose to apply coverage filters after the pipeline was run because it is much more convenient in this type of exploratory data analysis.
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CEO of Professional Science Editing for Scientists @ prosciediting.com