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Assembled contigs with excessive coverage discrepancies ≥3,000 were discarded.
Obviously, coverage discrepancies between different databases can result in variations of analytical outcomes.
Coverage discrepancies can result from the use of specific genome amplification methods [ 4] (e.g. GenomiPhi, Repli-G, PCR), potentially differentially representing areas of the genome.
To assemble the high-coverage strain, the lower-coverage duplicates were removed, GapFiller was run again, then finally reads were mapped back to the contigs and the final assembly was inspected in tablet to check for coverage discrepancies, duplicates, Ns, and unpaired reads.
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Violation of the condition in (3) can happen due to the coverage discrepancy between the sampling intervals of two sensors.
Additionally, fragment length normalisation was performed in order to eliminate potential bias in coverage resulting from discrepancies in the distribution of fragment lengths between samples.
In Section 4, the proposed method is evaluated with a realistic application scenario and is analyzed with nonideal problems such as the discrepancy between approximated and actual identification sensor coverage and variance of actual identification sensor coverage.
In- hospital attending physician coverage attenuated this discrepancy between weekdays versus nights and weekends but was not fully protective for tracheal intubation-associated events.
Ryan balked at news coverage noting the discrepancy, tweeting, "Associated Press is wrong.
As the genome wide coverage increases the discrepancy in callable bases between the two library preparation methods decreases.
dsDNA, double-stranded deoxyribonucleic acid; SCE, Subspace Clustering Error; RCE, Row Clustering Error; CI, Coverage Index; DNC, Discrepancy in the Number of Clusters; PPT, Phage Proteomic Tree; ICTV, International Committee on Taxonomy of Viruses.
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