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As an interdisciplinary field, topics of this journal cover tissue engineering, regenerative medicine, mechanical devices from the perspectives of materials, biology, medicine and mechanical engineering, with a focus on manufacturing science and technology to fulfil the requirement of bio-design.
Needless to say, our assessment for tissue heterogeneity does not completely cover tissue heterogeneity in the tumor.
Cover tissue sections with 3-4 drofs of protein block buffer (DAKO) and incubate slides for 15 minutes in humid chamber at room temperature.
After blocked with normal goat serum, diluted bcl-xl and cyclin D1 primary antibodies (at a dilution of 1 300) were added to completely cover tissue and incubated in humidified chamber overnight at 4°C, and then incubated with biotinylated second antibody, and finally incubated with streptavidin-peroxidase conjugate (S-A/HRP).
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Nipping in with her mobile phone camera and raiding the kind of things you find in airline lavs (disposable seat covers, tissues, etc), Katchadourian photographs herself done up like a 15th-century Flemish woman.
You might, however, want to cover the tissue boxes with white, brown, or yellow, construction paper before you begin.
In the present case, we used a gracilis muscle flap to cover the tissue loss area.
Use of the CKα antibody can be extended to protein microarrays that cover more tissue types and samples [27], [28].
Cover the tissue slides with cover glass and seal with paper cement.
The culture medium was amended to cover the tissue at the next day.
Add 100 μl probe solution from above step on each section to cover the tissue properly.
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