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Prolong Gold antifade containing DAPI for nuclear staining (Invitrogen) was used to cover the sections.
For the labelling reaction, a TdT buffer solution (composition: potassium cacodylate 100 mmol l−1; cobalt chloride 2 mmol l−1; dithiothreitol 0.2 mmol l−1; pH 7.2) containing 0.3 U μl−1 biotinylated dUTP was added to cover the sections, incubated at 37°C for 60 min in a humidified chamber.
Briefly, slides were placed in Coplin jars containing enough 0.01 M sodium citrate solution (pH 6.0) to cover the sections, then autoclaved at 121°C for 10 minutes in the case of Her-2, and 15 minutes for both ER and PR.
Ensure you have enough fabric to cover the sections of the dress between hems.
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Although some of the officers who were allowed to go home had been patrolling Central Park South, officials have said that none were assigned to cover the section of the park where the majority of the attacks occurred.
In principle, the physical time needed to cover the section is not affected, but the discomfort per unit of time increases with density.
One hundred microliters of reaction mix per slide will adequately cover the section.
This chapter covers the sections and elements required in the various types of documents that a postgraduate may have to prepare.
Cut ~19" strips to cover the middle section (the section with the side panels).
High-tension nets cover the electronic sections to protect them from home run balls.
If your shin guards have ankle pads, they should cover the bony sections on both sides of your ankle.
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CEO of Professional Science Editing for Scientists @ prosciediting.com