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Finally the mixture is spin-coated onto a nitrogen-plasma cleaned cover slides.
Following, to spread double distilled water suspensions of cells onto glass cover slides effectively, all glass cover slides were pretreated with poly-l-lysine (Sigma) and air dried at room temperature performed in our NSOM imaging study.
Before each experiment, the surface of the glass cover slides (13 mm diameter and 1.2 mm thick) was treated with a cleaning solution (Korenblum et al. 2008).
Various substrates like glass cover slides, TEM grids, and (111) p-type silicon wafers were used to facilitate various types of NP characterization.
NPs were directly deposited, at room temperature and under high vacuum, on glass cover slides, TEM grids and (111) p-type silicon wafers.
Cells were cultured on round cover slides for 24 h.
Cover slides were stained with DAPI and mounting solution.
Cover slides were mounted in Fluorsave mounting media (Calbiochem).
Cells were cultured on 12 mm poly-lysine coated glass cover slides.
For most FRET measurements via fluorescence microscopy samples are fixed and mounted on cover slides.
Cover slides were analyzed by fluorescence microscopy (Leica DMRXA fluorescence microscope).
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