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In selecting these time courses for further analysis, we considered those components involving regions known to mediate brain response to pain [4] and showing a consistent signal increase (activation) coinciding with each pain stimulation block, irrespectively of the duration of the activation.
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Genes whose expression levels increased or decreased by a factor of three or more from their time-zero levels, in at least 3 of the time-course samples, were selected for further analysis.
Serum hCG levels were sorted within each MTX chemotherapy course number and data were excluded for further analysis from the moment therapy other than MTX started (hysterectomy, second curettage and second-line chemotherapy).
Considering the effect of intended weight reduction, we excluded 142 individuals who reported participation in weight-reduction courses during the follow-up, leaving 440 individuals for further analysis.
In order to perform statistical analyses of this activity, time courses at the peak frequency of the response were selected for further analysis [7].
For further analysis of gene modulation, we performed time-course quantitative RT-PCR on several genes: AXL, UBC, FBXL2 and SLC12A5 (figure 2).
Mice were macroscopically monitored for the development of papillomas during the course of the experiment and were sacrificed after week 21 when tissues were collected for further analysis.
The time courses of the estimated currents for each dipole within these selected regions were extracted and used for further analysis.
For further analysis of PPARγ methylation in adipocytes, we next investigated the time course of promoter demethylation during adipocyte differentiation of 3T3-L1 cells.
The cases were preselected by histologic criteria for further analysis, and samples were taken from patients who died from acute influenza after clinical courses of <1 week (16 – 16 ).
They were then taken to a laboratory for further analysis.
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