"It needs to be sorted, of course it does.
We also monitored the population doublings of the Sca-1+ and Sca-1− sorted cells over the course of 5 weeks.
However, based on a time course for induction of gene expression in sorted Akt1-GFP+ and Akt1-GFP− populations (+/− Ly, +/− Tam), it appeared that Akt1 also had cell autonomous effects on Snai1 and Sox17 transcription.
(C ) Gel electrophoresis of time-course (0′ 5′) microccocal nuclease (MNase) digestions using sorted His C mutant and wild type sibling embryos at 5.5 6.5 hr after egg laying, respectively.
All time courses of red/green ratios for all ESTs were sorted via self-organizing maps with Euclidian distance [ 21, 22] (25 cluster (5 × 5) neuron map).
These relationships: I-you distant provider dominance, I-you blurred sympathy, and I-you sorted mutuality were presented at the first course afternoon.
Over the course of 14 days in culture, the relative abundance of sorted MaSC/basal cells transduced with virus-encoded shRNA-GFP versus a reference population of MaSC/basal cells transduced with a virus-encoded mCherry fluorescent protein was measured by flow cytometry.
As requested, we performed time-course micrococcal nuclease (MNase) digestion experiments with chromatin isolated from sorted His C mutant and from wild type (sibling) embryos, respectively.
"Bigger brakes up front, suspension's sorted, no diff yet – well, it's got a diff of course".
