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The time course samples were collected out of series and in duplicate to minimize experimental variance.
The DU145, VCaP, RWPE, as well as the VCaP and LNCaP AS-sequenced time course samples were treated with DNAse.
The VCaP and LNCaP time course samples submitted for SMS, as well as the PrCa and PrCa-Adjacent normal samples, were not treated with DNAse during sample preparation.
Identical samples were submitted for SMS and AS sequencing in all cases with the exception of the VCaP and LnCaP time course samples.
To evaluate ROS changes during the time course, samples were stained with 10 µM DCFH-DA in the dark for 20 min at 37°C [31].
For the ES and GCNF−/− time course samples, we linearly interpolated between the mean expression values at each of the 4 time points, obtaining a 7-value time profile for each oligonucleotide probe.
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The solution was an appetizer course sampler of fish, pasta and salad.
A special of marinated chicken breasts in a sun-dried tomato pesto-caper sauce was the best main course sampled.
Visitors hike through rows of coffee trees, meet the pickers and, of course, sample fresh brews in the mill's cupping room.
So I'd tell a woman to ask herself, 'What is the subliminal image beyond my desire?' " Of course, sample sales often present perfectly sound temptations.
On a recent night at this West Village spot, a young woman — blond hair, petite, ebulliently extolling her recent wine-tasting course — sampled a glass of red.
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