The time course of transcript abundance during partial clubroot resistance response was monitored at the whole plant level, and direct comparisons between partial resistance and susceptibility responses were made using the same host genotype.
For each transcript that had significant differential expression between sleep and sleep deprivation, linear regression was used to fit the time course of transcript expression in unperturbed animals starting with baseline expression and continuing with expression in spontaneously sleeping mice sacrificed after 3, 6, 9 and 12 hours.
Due to non-linear changes in local velocity, the actual time-course of transcript density in a given tissue element was quite different from what could be expected intuitively from Figures 2A and 2B.
The time course analysis of transcript expression of the h4-h2b genes revealed that they were probably co-regulated compared to the h3-h2a transcripts.
Time course studies of transcript levels upon stress application together with the study of promoter expression in vitro and in planta are currently being undertaken to elucidate the regulatory mechanisms underlying the observed differences in transcript abundance.
To understand the regulation of the different XTH genes identified through the transcriptome sequencing, a time course study of transcript accumulation of various rose XTH genes was carried out by real-time RT PCR in 0.5 µL L−1 ethylene-treated petal AZs of R. bourboniana and R. hybrida as well as under conditions of natural abscission.
Baugh et al.[ 13] developed a preliminary, biological model for the C lineage regulatory network based on a developmental time course of gene transcript abundance data from animals wild type for all genes in the network.
A time course of LHY transcript accumulation showed that isoforms with a retained intron in the 3' UTR (I9R) and the fully spliced form (I9S) peaked at the dark/light transition period (ZT0, Figure 3B).
To screen for genes involved in muscle recovery growth, we undertook a time-course analysis of transcript expression in muscle of trout fasted for one month and then refed for 4, 7, 11 and 36 days.
In this study, to obtain more information on the system-wide integration of recovery growth in muscle, we undertook a time-course analysis of transcript expression in trout subjected to a food deprivation-refeeding sequence.
Consistent with the time-course analyses of transcript profiles and functional categories, the most dramatic changes in expression were noticed in the transition from Day0 → Day5, where 173 transcripts were specifically down-regulated and 78 transcripts were up-regulated, and in the transition from Day15 → Day25, where 280 and 139 transcripts were specifically up and down-regulated, respectively.
