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The two isolates did not share the same MLVA type which could be the result of mutation events in the course of outbreak.
Since a formal randomized trial of seed disinfection in the community would be unethical, our understanding of the usefulness of disinfection is based largely on observations made in the course of outbreak investigations.
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Subsequently, in partnership with WHO, another set of studies have examined the relative roles of access and acceptance on use of influenza vaccine over the course of outbreaks during and after the pandemic of 2009 in Pune, India.
Organism specific MLST analysis allows more definitive identification of bacterial isolates providing useful epidemiological data for identifying the source and course of outbreaks.
Moreover, answers can be accurate if no stochastic event occurs that could substantially alter the course of outbreaks.
In the situation described here, thorough outbreak investigation was complicated as a result of continuation of trips through different countries during the course of outbreaks.
High-throughput sequencing successfully proved to be a valuable tool for tracing resistance plasmids in the course of outbreaks as well [ 87].
This set of strains, collected from patients over the course of outbreaks or as sporadic cases in endemic regions, encompasses every region in which the main pandemics took place [ 3].
Also needed is the ability to translate the data into relevant information enabling microbiologists, clinicians and public-health epidemiologists to implement control measures in real-time and alter the course of outbreaks.
The major challenge is to make WGS a genotyping tool during the course of outbreaks, and for this it will be necessary to facilitate access to sequencing platforms.> -wrap-foot> aAbbreviatioNA: NA, data not available.
More research, however, is needed to establish in more detail how changes in the course of outbreaks in and outside the country and media coverage are related to risk perceptions and preventive behaviour.
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