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The expression of the target proteins using the same vectors in vitro showed that differences in expression level were wholly dependent on the environment of the expressing cell and an investigation of the time course of expression showed it could affect substantially the observed expression level for poorly but not well-expressed proteins.
Antibodies constitute a potent tool to study such proteins, enabling conclusions on protein location and time course of expression.
This extremely low value of the cake moisture content implies that the liquid contained within the microorganism cells was partially removed by the mechanical pressure when the ultrahigh pressure is applied to the cake in the course of expression.
The understanding of the time course of expression of cerebrospinal fluid (CSF) biomarker levels following severe TBI is an important step toward using these biomarkers to measure injury severity and/or early response to therapeutic interventions.
Reverse transcription-polymerase chain reaction (RT-PCR) is used in the present study to describe the time course of expression of the family members during embryonic development, and their expression in adult tissues.
We then evaluated the time course of expression of select chaperones in response to heat shock.
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Thus the skewed time course of expressions of genes in each cluster is characterized by a different vector of Fourier coefficients β and associated variance σ.
GFP expression is highly dynamic throughout the developmentally staged EST libraries, and each expressed GFP shows a distinct time-course of expression (Fig. 2).
We also provide a time-course of expression that we used to characterize the transcriptional regulation of the gene families studied.
10.7554/eLife.03743.012 Figure 3. Time-course of expression of Pitx2 in whole embryos and in isolated anterior halves, and comparison with cVg1.
The time-course of expression of flavonoid hydroxylase genes in the red-skinned cultivar 'Merlot' revealed that gene VvF3'H-1 is expressed throughout ripening.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com