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In contrast to traditional local co-variance techniques, DCA prunes the covariance values by separating out direct statistical couplings from correlations that arise indirectly through coupling chains (see Fig. S1), thereby vastly improving the accuracy of contact residue predictions.
"Romeo and Juliet" laws in several American states exempt close-in-age couplings from prosecution.
Bouvignies, G., Meier, S., Grzesiek, S. & Blackledge, M. Ultrahigh-resolution backbone structure of perdeuterated protein GB1 using residual dipolar couplings from two alignment media.
In addition to basic utilities to solve for order tensors and back calculate couplings from a given order tensor and proposed structure, a number of improved algorithms have been incorporated.
Here, the observation of residual dipolar couplings from alpha-methyl mannose bound to mannose binding-protein in a dilute liquid crystalline medium has allowed the determination of a bound ligand's average orientation.
The absence of additional 2JC-H and/or 3JC-H couplings from the protons associated with these carbons (1H = 1.63 and 1.69 ppm) suggests that these structures are terminal.
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The matrix H has constant columns, except for the diagonal, which reflects self-coupling from each cell onto itself.
This is a reasonable assumption if we conceptualize each firing rate unit (x_{j}) as corresponding to an individual neuron; whereas neurons can have self-synapses (or autapses [24]), refractory dynamics would tend to suppress self-coupling from influencing the firing rate.
ProQM-resample was benchmarked on the independent EVfold_membrane data set (Hopf et al., 2012) consisting of 15 340 models for 25 targets generated with CNS (Brunger et al., 1998) using distance constraints from evolutionary couplings extracted from large multiple sequence alignments (the set is actually larger, but only 25 targets had a known structure).
At a microscopic level, such couplings result from interactions of phonons with other phonons (phonon-phonon interaction from anharmonicity), or with charge carriers (electron-phonon interaction).
However, these couplings far from behaving in a linear way, show a complex behavior that sometimes is difficult to evaluate.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com