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The coupling time of pyrene-modified C3 spacer was extended to 15 min. Oligonucleotides were deprotected in concentrated ammonia at 55°C for 12 h.
Temperature was controlled using a weak coupling to a bath of constant T (300K; coupling time of 0.1 ps) and pressure by a weak coupling to a bath of constant P (1 atm, coupling time of 0.5 ps) with the Berendsen method [36].
The Berendsen coupling was employed to maintain a constant temperature of 310 K and constant semiisotropic pressure of 1 bar with coupling time of 0.1 ps and 1 ps respectively.
A constant temperature of 300 K was maintained using a Berendsen thermostat with a coupling time of 0.1 ps.
The velocity limit was set to 10. Berendsen temperature control with a coupling time of 1 ps was utilized in all simulated annealing calculations.
The DNA sequences on the microarrays in this project were synthesized with a light exposure dose of 4.5 J/cm, with coupling time of 40 s at monomer concentrations of 30 mM.
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Fig. 1 Schematic of the MOTion trap and the radially coupled time-of-flight mass spectrometer.
This distinctive feature allows couples times of PRF extension.
Do this a couple times of times each day.
Do it a couple times, of course if they make problems.
matrix assisted laser desorption/ionization coupled to time-of-flight.
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