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The initial study of centromere coupling reported that in chromosome spreads exhibiting centromere coupling, Zip1 foci and kinetochore pairs occur in approximately equal numbers (about sixteen) and exhibit a high level of co-localization [8].
These results, obtained in native tissues, are consistent with normal electrical coupling reported in transfected HEK293 cells overexpressing the mutant Cx30T5M protein (22).
Moreover, eq 10.18a yields the Marcus and DKL results, except for the additional explicit expression of the coupling reported in eqs 10.18b and 10.18c.
NHS-labeled MB was conjugated to the 5′-end of amino-modified TGF-β1 aptamer through the succinimide ester coupling reported previously.
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