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Main chain assembly was mediated by diisopropylcarbodiimide (DIC) and coupling completion (45 to 60 min) was assessed by Kaiser's test.
Fmoc amino acids were coupled in the presence of PyBOP and NMM for 30 min. Each step was repeated twice to ensure coupling completion.
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Components of the ESCRT complexes have also been implicated, either directly or indirectly, in certain receptor recycling pathways [ 8,60,61], but further studies are required to determine whether these interactions might also couple completion of recycling to fusion competence.
These rearrangements underlie the coupling between completion of lid assembly and licensing of the lid for attachment to the base.
In this study, through a gain-of-function genetic screen, we identify the novel regulator VosA that couples the completion of sporogenesis and focal accumulation of trehalose in spores.
In the starvation-induced developmental program, induction of sporulation is normally coupled to completion of aggregation.
"A couple more completions, and we would've been talking about a great comeback victory," Rutgers Coach Greg Schiano said.
This change removes the contribution of previous elongation activity when the gene re-activates, and implements a simple coupling between the completion of elongation (which we equate with the production of a mRNA) and the state of the promoter.
These examples indicate that in semelparous forms, in which full vigour and function are required until virtually the end of life, senescence has an onset closely coupled with the completion of the reproductive process and is governed by relatively simple enzymatic mechanisms that can be modified by natural selection.
High enrollment in primary schools is often coupled with low completion rates in secondary and postsecondary education.
New industrial parks and office parks were being constructed within a couple years of completion of the new parkway along NY 9A.
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