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The use of IRES sequence in bicistronic constructs eliminates promoter interference problems and directly couples the expression of a reporter gene to the expression of the gene of interest [ 10].
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The conversion of ATP-DnaA to ADP-DnaA thus seems to couple the expression of nrdAB to chromosome replication [9].
The participation of Rb and E2F1 in the formation of the multiprotein complex that binds origins of DNA replication in mammalian cells appears to represent an effective mechanism to couple the expression of genes required for cell cycle progression to the activation of DNA replication.
And as anyone who has spent much time in -- or just in earshot of -- a typical couple knows, the "expression of needs" is often the Trojan horse of intimate warfare, since expressing needs means, by definition, that one's partner has thus far failed to meet them.
However, to date, investigators have relied on inherent interactions between the virus and the immune system, often coupled to the expression of a single cytokine transgene.
In Sinorhizobium meliloti, the FixL FixJ couple controls the expression of the nitrogen fixation genes through the binding of the two-domains response regulator FixJ to the fixK and nifA promoters.
An intriguing example of the coupling among the expression machineries is the observation that RNA splicing influences many subsequent steps of mRNA metabolism such as nucleocytoplasmic export [9].
The approach can be further applied to create a system in which the control of a gene network may be coupled with the expression of a recombinant protein.
Most importantly, we here show that the LPA-LPA1-Egr-1 axis is tightly coupled with the expression of periostin.
Increased TEER, coupled with the expression of tight junction associated molecules, represent unique characteristics of specialized barrier-forming endothelial cells.
The distribution of proteins involved in the recirculation of divalent metal ions leads us also to believe that ferroportin is coupled to the expression of DMT1.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com