All the heteroatoms coupled with proteins including water molecules, bound ligands and any co-crystallized solvent were discarded from the PDB file and the missing assignments like proper bonds, bond orders, hybridization and charges were assigned using the Molegro Virtual Viewer [40].
In addition, an iTRAQ-based proteomic approach coupled with bioinformatics, including GO and KEGG analyses, were also applied.
Following this, an integrated CFD study of header coupled with sodium pump including impeller and diffuser has been carried out.
"Those areas over which we have concerns will see additional forces flowing into them". Petraeus also said Thursday that military efforts need to be coupled with political reforms, including the absorption of what he termed "reconcilable" outlaw groups into society.
He says a public-health model will be fully effective only if coupled with other efforts, including more creative policing and efforts to get gang members back to school or to work.
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The arrays are coupled with digital hardware including time-to-digital converters (TDCs) capable of resolutions better than 25 ps and recharge periods shorter than 7.5 ns.
However, if energy projects aim at a higher productive outcome of rural communities, electrification programs need to be coupled with complementary infrastructure, including training and education [45].
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Thus, analytical strategies that are coupled with efficient methods, including enrichment, solubilization, and digestion of membrane proteins and N-glycoproteins, must be formulated.
