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Importantly, WT-FIH exhibited fully coupled turnover, such that O2 activation always led to substrate hydroxylation.
This work establishes that proper positioning of the primary substrate (CTAD) is required to support coupled turnover by FIH.
The overall trend in the kinetic parameters measured by coupled turnover (Table 2) was dominated by bulk, as the kinetic parameters for the Gln → X point variants decreased monotonically in a series: X = (Ala > Asn > Glu and His ≫ Leu).
The ordered sequential consensus mechanism for αKG oxygenases leads to coupled turnover when primary substrate binding stimulates reactivity toward O2, a phenomenon termed substrate-induced activity enhancement, priming, or triggering by different groups.
However, the coupling ratio in D2O for Gln → Ala CC = 1.4 ± 0.2) and Gln → Asn (C = 2.2 ± 0.2) approached unity, indicating that solvent deuteration led to more tightly coupled turnover for these variants.
Similar(55)
Syncrip is important for mRNA processing, translation coupled mRNA turnover and splicing [ 32].
We showed that, at the sub-cellular level, the variations in junctional forces can be coupled to turnover of E-cadherin-GFP at the junctions.
Additional links to AP1 were identified via several components of a multi subunit autoregulatory ribonucleoprotein complex, potentially involved in translationally coupled mRNA turnover [ 41].
These contradictory observations can be reconciled by the observation that the concurrent expression of synthesizing and degrading enzymes has the strongest impact on tumor growth and metastasis, suggesting that increased synthesis coupled to turnover of HA is decisive in this context.
Future studies will be needed to definitively establish the full paths of the leading- and lagging-strand DNA bound to the CMG and the extent to which the disparate AAA+ and RecA-family ring-translocases share or diverge in coupling ATP turnover to specific subunit movements that drive substrate translocation.
Philly did, and we had a couple turnovers early".
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