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The coupled procedure aimed at predicting the detailed structural damage together with reasonable global ship motions.
The coupled procedure allows us to consider uncertainties during the crack growth process.
A natural barrier that can be remarked in this type of approach is the numerical error due to the finite difference procedure, which may affect the convergence of the coupled procedure, as well as the precision of the solution, especially for nonlinear transient phenomena.
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Recombinant SC142 scFv was coupled with biotin at concentrations of 100 μg ml−1 in PBS by the N-hydroxysuccinimide (NHS) esters coupling procedure described by the manufacturer (Pierce).
Ahmed H. Zewail and co-workers at Caltech coupled a procedure for generating three-dimensional electron microscopy images with ultrafast measurement methods.
Gpx4 activity was measured by the coupled assay procedure using 78 μM phosphatidylcholine hydroperoxide, its specific substrate [ 5].
Gpx1 activity in liver, kidney and red blood cells and Gpx3 activity in plasma were measured by the coupled assay procedure using 120 μM H2O2 [ 48].
This obviously demands a further coupling procedure.
Figure 7 Illustration of iterative coupling procedure.
The two-way coupling procedure is employed.
The atmosphere-ocean coupling procedure is described next.
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