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For litter counts, samples were collected at a variety of points within the shed, the litter was well mixed, a sample was weighed, water was added and left overnight at 4°C.
For CFU counts the samples were immediately plated on TSA plates and for PFU counts samples were immediately plated using the method described above for phage titration.
For viability counts, samples were diluted in 0.9% NaCl, spread or spotted on LB plates and incubated 12-16 hours before colony counts were taken.
We first summarize the raw data contained in a fastq file into a matrix of counts (samples in the columns, hairpins in the rows) with options for allowing mismatches and small shifts in hairpin position.
For determination of CD34+ cell counts, samples were collected on days 1 (predose), 3 (6 h post dose), 5 (6 h post dose), 7 (48 h post dose), and 10 (120 h post dose).
Lower inter-reader agreement for CTC definition was observed for CellSearch® images of samples from patients with M0 disease, with low CTC counts, samples from the IJB dataset, and samples drawn after the administration of systemic treatment.
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"Aerial counts, roadside counts, sampling methods, dung counts: It all works very well for prey, but not for lions," he says.
To standardize sequence counts across samples, samples with <5000 sequences per sample were removed.
When liquid samples are involved, this effect can usually be minimized by consistently counting samples of equal volume.
However, since transect surveys encompassed 4.3 times as much area as point counts, observed densities averaged about three times higher for point count samples.
A total of 2323 phytoplankton cell count samples collected by the Florida Fish and Wildlife Conservation Commission (FWC) matched valid ocean color satellite observations over this period.
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