Latitude of origin was estimated based on the reported city and country of collection provided by the Fungal Genetics Stock Center [26].
For this purpose, we partition globally sampled sequences based on their country of collection.
Annotations include isolate name, subtype and/or lineage, passage history, year of collection, submission date, host and country of collection.
We found a significant correlation between cluster assignment and both host and country of collection, but did not find a correlation between cluster assignment and collection date or subspecies assignment.
Although locality information on older specimens may only contain the county or even country of collection, more recent specimens often include a detailed description of the collection site (e.g., location near a highway or cross streets) or highly accurate GPS coordinates.
Seeds were collected from each of 62 individual plants along with material for a herbarium and for DNA extraction (both from 42 of the 62 plants): all samples were named with letters indicating the country of collection and numbers indicating the order of sampling (Table 1).
Detailed list of the 209 Osprey samples indicating: sample lab code, subspecies according to morphology classification, country of collection, locality, sample type (tp = toepad; wb = wet blood; db = dry blood; ft = feather; fs = fasta sequence), codes, gene bank number accession for cyt b and ND2) and name of the institution and/or collector (with affiliation).
A full listing of all varieties included in this study together with information on their country of production, collection point and type can be found in Additional file 2. Summary statistics for each sample collection including the count of varieties, samples and the frequency of samples per country of production are shown in Table 1-A.
Patients were diagnosed, staged and treated for HAT according to the guidelines of the national sleeping sickness control program of the country of sample collection.
Comparison of the levels of the markers between stage 1 (S1) and stage 2 (S2) T.b. rhodesiense patients classified according to the country of sample collection.
