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Under blinded conditions, cell/foci counting were performed until at least 40 cells and 40 foci were registered in every sample.
Sectioning and cell counting were performed by separate investigators who were blinded with respect to the group to which the animals belonged.
Staining and counting were performed using the same methods as the apoptosis evaluation.
Immunostaining and counting were performed as previously described (Parkinson et al., 2001, 2003, 2004, 2008).
Tissue damage evaluation and cell counting were performed by an investigator blinded to the experimental groups.
All other analyses and preparations of smears for PMN counting were performed within 6 h.
Similar(37)
Limb counting was performed in 28 animals.
Counting was performed using a standard sampling procedure.
Counting was performed without knowledge of treatment.
Oocyst counting was performed using a McMaster flotation chamber.
The counting was performed in a blinded manner.
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