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To avoid experimenter bias, immunohistochemical analysis and counting were conducted by an independent person blinded to the adiponectin status.
On day 21, bronchial responsiveness tests, bronchoalveolar lavage, and cell counting were conducted.
Experiments with inert aerosol particles followed by time-resolved particle counting were conducted in the prototype room for comparison with the CFD observations.
Tg(hand2 EGFP) pd24 larvae were treated and processed as described (Yin et al., 2012) by imaging on a Zeiss Pascal confocal microscope, and image processing and cell counting were conducted using Fiji (http://fiji.sc/Fiji).sc/Fiji
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Counting was conducted by an experimenter who was blind with respect to group assignment.
Opposition parties and independent election observers have complained that government authorities have denied their access to the command center where the final stage of vote counting is conducted.
The 1990 census counted about 6,100 homeless people on the streets of Manhattan, and the 2000 census never released any figures, in part because of criticism of how the 1990 counting was conducted.
Varve counting was conducted based on soft X-ray images to construct an age model for the SG12-LM3 core.
Tritium counting was conducted (at Geo-Science Laboratory Co. Ltd, Nagoya, Japan) with a low-background liquid scintillation counter, Aloka model LB5 following electrolytic enrichment of 3H by a factor of about 25 using Fe Ni electrodes.
Automated counting was conducted for Ki67/Olig2 stained cells.
The counting was conducted as described previously [ 39].
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