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The counting of TUNEL-positive cells was repeated three times, and the percentage from each counting was calculated.
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Error bars and statistical significance of invagination counting were calculated using the Poisson distribution [53].
The total number of TH- stained neurons and nissl counts was calculated as described24,25,26.
The sgRNA count was calculated using a custom script in Python (version 2.7.3) and Bland Altman MA plot was generated using R (version 3.1.1).
The maximum count in each lesion ROI was obtained and a geometric mean (GM) of the two counts was calculated.
In reality, if the medal count was calculated like a grade point average, the US would be in third with Norway being in second place.
This was done in triplicate and the average of the three counts was calculated.
Estimated Optical Fractionator count was calculated as the sum of cells in all five regions.
The absolute CD8+ T cell count was calculated by multiplying the absolute lymphocyte count by the CD8+ percentage.
The absolute lymphocyte count was calculated by multiplying the white blood cell count (#/ml) by the lymphocyte percentage.
For proteins that were identified in both experiments, the ratio of normalized spectrum counts was calculated and then log-transformed.
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CEO of Professional Science Editing for Scientists @ prosciediting.com