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At the end of the exposure time, coupons were neutralized, sonicated, serially diluted and plated as described above; the resulting TSA plates were incubated at 37°C with colony counting performed 24 h after neutralization.
Each experiment was repeated twice, with each counting performed in quadruplicate.
The cell counting performed in both models (TAA and CCl4) of liver damage showed a significant increase of the total number of ED1+/COX-2+ cells after 24 h (1.4-fold) as well as after 48 h (1.86-fold).
Cells were harvested post-ADI-PEG20 at days 2 4 with cell line counting performed using a Beckman Vi-Cell cell viability analyser (Beckman Coulter Ltd ,High Wycombe, UK).
A statistical counting, performed with >150 cells, demonstrated that ≈30% of bacteria (±3%, p = 0.95) contained large electron-dense granules in the cytosol (arrow in Fig. 5a).
Lamar, the unrivaled king of L.A. hip-hop for a generation and counting, performed, produced and curated the soundtrack, which pops up in album, record and song of the year (for his SZA collaboration "All the Stars"), along with rap song (for "King's Dead," with Jay Rock, Future and James Blake) and song written for visual media.
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Generally, clinic-based pill counts performed poorly in other studies, also [ 42].
The Aber Valley Male Voice Choir was founded in 1959 and counts performing at the Houses of Parliament as one of their many highlights.
The recovered fluid was pooled and filtered over sterile gauze, and a total cell count performed.
† Differential count performed in 608 patiens.
HIV infected persons had a CD4 count performed.
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