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Of course it is possible that targets could be met via creative strategies such as the use of offsets or the counting of emissions reductions in gases other than carbon dioxide.
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In each iteration, a new set of parameter values is derived from the counts of emissions and transitions in the Viterbi paths [ 17] of the training sequences.
In each iteration of Viterbi training, a new set of parameter values ϕ is derived from the counts of emissions and transitions in the Viterbi paths Π∗ for the set of training sequences X.
In each iteration, a new set of parameter values is derived from the estimated number of counts of emissions and transitions by considering all possible state paths (rather than only a single Viterbi path) for every training sequence.
The system was calibrated prior to each experiment and 10,000 events analysed for each sample with FACS Diva Software BD Biosciencess) using control based setting of thresholds and single cell counting of fluorescent emissions at 530nm for FITC labelled Annexin V binding and greater than 575nm for propidium iodide staining.
Also, it will be important to identify means for the ITMOs to be tracked in order to avoid double-counting of emissions reductions.
Effect of lesion contrast and number of emission counts were also investigated.
The effect of contrast and number of emission counts was similar to the physical phantom.
To produce guidelines to assist selection of the best method for a specific data type, the effects of different data parameters including lesion size, lesion contrast, reconstruction protocol and number of emission counts have been studied.
From our results, lesion size and contrast had the most impact on method selection whereas reconstruction parameters and number of emission counts had a lower impact but they can be used to fine tune the accuracy.
Minor points: 1) As the authors correctly point out, the accurate counting of molecules, as distinguished from counting fluorescent emissions or "events", is very difficult by localization microscopy.
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