Sentence examples for counting manual from inspiring English sources
Cell counting, manual or electronic, is essential to determine growth rates accurately and to set up reproducible experiments.
Ki-67 immunohistochemistry is also limited by low reproducibility between laboratories, ongoing debate over both the optimal antibody for testing and the method for cell counting (manual versus automated), in addition to potential problems resulting from tumor heterogeneity [ 38].
To determine whether the observed yield differences between the CEK and CPK methods are due to differences in the method of cell counting (manual vs semi-automatic), we compared CTC counts obtained using the automated CEK method with that of a manual cell count of the same sample.
Using our algorithm, total computing time for 47 images was 14.5 seconds, which was 596 times faster than manual counting [total manual counting time for 47 images = 8640 seconds (2.4 hours)].
Repeat counts (manual or automated) that are averaged and properly calibrated pipettes are therefore vital.
To test the reliability of the cell count, manual counts using a hemocytometer were performed.
To facilitate convergence, we carried out the parameter estimation procedure in stages of increasing model complexity, as recommended in the Count manual [ 24].
While especially for high cell counts, the manual counting showed a deviation of up to 400 cells/mm2 (41% variation), algorithm-determined T cell numbers generally lay in between the manually observed cell numbers but with perfect reproducibility.
Results: The median nucleated red blood cell counts/100 white blood cell counts for manual microscopy in umbilical cord blood; placental samples were 7.5 and 3.0, respectively (P <.0001).
After staining, TRAP-positive multinucleated (nuclei ≥ 3) cells were counted using manual counting or a nuclei counter plug-in in the Image J program.
In addition calculation of the absolute deviation of algorithm counts from manual counts gives a more transparent quantification of errors.
