For glucose and blood count determination, blood was drawn into S-Monovette® tubes with K3-EDTA.
All the vessels counted determinations were performed by the same pathologist blinded from SUV data.
Dispersions were evaluated by dynamic light-scattering for zeta-average diameter (Dz) and zeta-potential analysis; dialysis for determination of drug entrapment efficiency; plating and CFU counting for determination of cell viability of Mycobacterium smegmatis or tuberculosis, minimal bactericidal concentration (MBC) and synergism index for DODAB/drug combinations.
A small aliquot was removed, mechanically dissociated with a 200 μL micropipet, and used for counting and determination of cell density.
NIR spectroscopy describes informative patterns in application for somatic cell count determination, distinguishing of healthy and mastitis milk as well as in detection the presence and growth of specific bacteria, based on milk chemical composition changes and their influence on water spectral pattern.
Viruses isolated from a portion of the blood sample that was used for CD4 count determination were used for the neutralization studies described below.
Twenty-seven patients did not have a CD4 cell count determination made after 24 weeks for the following reasons: 17 patients either died, were lost to follow-up or transferred to another institution and 10 patients either did not have CD4 counts ordered by the physician or there was insufficient blood volume.
While the instrumentation required might seem prohibitively costly for routine use in many areas of the world where the incidence of tuberculosis is high, if the HIV infection rates are also high, flow cytometry may have been previously established for CD4 count determination; the province of KwaZulu-Natal in South Africa is one example of this.
Samples from liquid cultures and plates were collected at different time points for cell count determination.
The CD4 cell count determination was done using Cyflow counter (Cyflow counter, Partec, GmbH, Gorlitz, Germany).
White blood cell count determination was made using a Coulter Counter.
